Search results for "solution NMR"

1H, 13C, and 15N NMR chemical shift assignment of the complex formed by the first EPEC EspF repeat and N-WASP GTPase binding domain

2021

AbstractLEE-encoded effector EspF (EspF) is an effector protein part of enteropathogenic Escherichia coli’s (EPEC’s) arsenal for intestinal infection. This intrinsically disordered protein contains three highly conserved repeats which together compose over half of the protein’s complete amino acid sequence. EPEC uses EspF to hijack host proteins in order to promote infection. In the attack EspF is translocated, together with other effector proteins, to host cell via type III secretion system. Inside host EspF stimulates actin polymerization by interacting with Neural Wiskott-Aldrich syndrome protein (N-WASP), a regulator in actin polymerization machinery. It is presumed that EspF acts by di…

Structure of SNX9 SH3 in complex with a viral ligand reveals the molecular basis of its unique specificity for alanine-containing class I SH3 motifs

2021

Class I SH3 domain-binding motifs generally comply with the consensus sequence [R/K]x0PxxP, the hydrophobic residue 0 being proline or leucine. We have studied the unusual 0 = Ala-specificity of SNX9 SH3 by determining its complex structure with a peptide present in eastern equine encephalitis virus (EEEV) nsP3. The structure revealed the length and composition of the n-Src loop as important factors determining specificity. We also compared the affinities of EEEV nsP3 peptide, its mutants, and cellular ligands to SNX9 SH3. These data suggest that nsP3 has evolved to minimize reduction of conformational entropy upon binding, hence acquiring stronger affinity, enabling takeover of SNX9. The R…

Buchbesprechung: High-Resolution NMR Techniques in Organic Chemistry. Von Timothy D. W. Claridge.

2001

Der Nachweis zellulärer Reaktionen im kondylären Kiefergelenkknorpel mittels NMR-Mikroskopie Grundlagenuntersuchungen für eine hochaufgelöste Kernspi…

1992

Das regionale Auflosungsvermogen moderner bildgebender Verfahren reicht heute noch nicht aus, um Veranderungen des mandibularen Gelenkknorpels, zum Beispiel bei funktionskieferorthopadischer Behandlung, klinisch zu objektivieren. In tierexperimentellen Untersuchungen am Kiefergelenk des Hausschweins wurden auf der Grundlage von H-NMR-relaxometrischen Messungen an kondylaren Knorpelproben NMR-mikroskopische Untersuchungen am intakten Kiefergelenkpraparat durchgefuhrt. Dabei gelang es, den mandibularen Gelenkknorpel bis hin zur zellularen Ebene darzustellen und Regenerationsprozesse als Spafolge eines operativen Eingriffs am Kiefergelenk nachzuweisen. Die Ergebnisse wurden hinichtlich ihrer B…

Effects of nucleotide binding to LmrA: A combined MAS-NMR and solution NMR study

2015

ABC transporters are fascinating examples of fine-tuned molecular machines that use the energy from ATP hydrolysis to translocate a multitude of substrates across biological membranes. While structural details have emerged on many members of this large protein superfamily, a number of functional details are still under debate. High resolution structures yield valuable insights into protein function, but it is the combination of structural, functional and dynamic insights that facilitates a complete understanding of the workings of their complex molecular mechanisms. NMR is a technique well-suited to investigate proteins in atomic resolution while taking their dynamic properties into account…

Structural and Functional Analysis of BBA03, Borrelia burgdorferi Competitive Advantage Promoting Outer Surface Lipoprotein

2020

BBA03 is a Borrelia burgdorferi outer surface lipoprotein encoded on one of the most conserved plasmids in Borrelia genome, linear plasmid 54 (lp54). Although many of its genes have been identified as contributing or essential for spirochete fitness in vivo, the majority of the proteins encoded on this plasmid have no known function and lack homologs in other organisms. In this paper, we report the solution NMR structure of the B. burgdorferi outer surface lipoprotein BBA03, which is known to provide a competitive advantage to the bacteria during the transmission from tick vector to mammalian host. BBA03 shows structural homology to other outer surface lipoproteins reflecting their genetic …